Research Highlights

Three-dimensional virtual histology enabled through cytoplasm-specific X-ray stain for microscopic and nanoscopic computed tomography

Research Highlights |


Busse, M., Müller, M., Kimm, M. A., Ferstl, S., Allner, S., Achterhold, K., Herzen, J. and Pfeiffer, F. Proceedings of the National Academy of Sciences of the United States of America, 115(10), 2293–2298 (2018). [https://doi.org/10.1073/pnas.1720862115]

 

Purpose: The comparative analysis of our CT data with conventional 2D histology highlights the future applicability of the cytoplasm-specific X-ray staining method for modern histological and histopathological investigations using laboratory X-ray CT devices.

 

Figure: CT slices of microCT (A and B) and nanoCT (C−F) data in comparison with the histological microscopic slides (G−J) derived from the same mouse kidney after application of the developed eosin-based staining protocol. These demonstrate in particular the compatibility with conventional histological methods.